Optimized Allele-Specific Real-Time PCR Assays for the Detection of Common Mutations in KRAS and BRAF
نویسندگان
چکیده
منابع مشابه
SensiScreen® KRAS exon 2-sensitive simplex and multiplex real-time PCR-based assays for detection of KRAS exon 2 mutations
Activating mutations in codon 12 and codon 13 of the KRAS (Kirsten rat sarcoma viral oncogene homolog) gene are implicated in the development of several human cancer types and influence their clinical evaluation, treatment and prognosis. Numerous different methods for KRAS genotyping are currently available displaying a wide range of sensitivities, time to answer and requirements for laboratory...
متن کاملcommon kras and braf mutations in colorectal cancer patients
background kras and braf gene mutations are considered as key events in carcinogenesis progression of colorectal cancer. given the importance of these gene mutations evaluations, especially in metastatic patients, in terms of determination of therapeutic strategies, we studied the prevalence of kras and braf mutations in tabriz city. materials and methods deoxyribonucleic acid (dna) extracted...
متن کاملOptimized Multiplex Detection of 7 KRAS Mutations by Taqman Allele-Specific qPCR
UNLABELLED Establishing the KRAS mutational status of tumor samples is essential to manage patients with colorectal or lung cancer, since these mutations preclude treatment with monoclonal anti-epidermal growth factor receptor (EGFR) antibodies. We report an inexpensive, rapid multiplex allele-specific qPCR method detecting the 7 most clinically relevant KRAS somatic mutations with concomitant ...
متن کاملDetection of Ethambutol-Resistant Associated Mutations in Mycobacterium tuberculosis Isolates from Iran Using Multiplex Allele-Specific PCR
Tuberculosis is a serious global public health problem and its high prevalence is stron gly associated with the enhancement of drug resistance. In this study we demonstrate a multiplex allele-specific polymerase chain reaction (MAS)-PCR assay to simultaneously detect mutations in the first and third bases of the embB gene codon 306 ATG in ethambutol (EMB) resistant isolates of Mycobacterium t...
متن کاملNovel real-time PCR assays for the specific detection of human infective Cryptosporidium species
Cryptosporidium is a parasite that causes the majority of waterborne protozoan outbreaks of gastrointestinal disease worldwide. Cryptosporidiosis usually presents as self-limiting, although often prolonged, diarrhea with abdominal pain, nausea, vomiting and low-grade fever. Infection can be life threatening in some severely immunocompromised groups. Post infection sequelae have also been report...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Journal of Molecular Diagnostics
سال: 2011
ISSN: 1525-1578
DOI: 10.1016/j.jmoldx.2010.11.007